| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
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| Autores: |
Alves,Clebert José
Figueiredo,Salomão Moreira de
Azevedo,Sérgio Santos de
Clementino,Inácio José
Keid,Lara Borges
Vasconcellos,Sílvio Arruda
Batista,Carolina de Sousa Américo
Rocha,Vivianne Cambuí Mesquita
Higino,Severino Silvano
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| Data: |
2010-06-01
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| Ano: |
2010
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| Palavras-chave: |
Brucella ovis
Isolation
PCR
Ovine
Paraíba State
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| Resumo: |
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822010000200016
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.41 n.2 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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